Takara Primary hepatocyte medium

Human primary hepatocytes (hphep) are an invaluable tool for academic and pharmaceutical labs studying liver disease pathology or as part of the drug development pipeline. To assess disease models and hepatocyte toxicity, robust and predictive hphep cell cultures are required. However, currently available culture media severely limits the utility of primary hepatocytes because hepatocytes lose their function and viability within a week.

Human primary hepatocytes (hphep) are an invaluable tool for academic and pharmaceutical labs studying liver disease pathology or as part of the drug development pipeline. To assess disease models and hepatocyte toxicity, robust and predictive hphep cell cultures are required. However, currently available culture media severely limits the utility of primary hepatocytes because hepatocytes lose their function and viability within a week. Additionally, daily feedings are required to maintain the hphep cultures.

Cellartis Power Primary HEP Medium is specially formulated to enable long-term maintenance of cryopreserved plateable hphep in conventional 2D cultures—cells are viable and maintain functionality for four weeks. This medium further simplifies culturing of hepatocytes with a weekend-free feeding schedule.

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Overview

• Extended culture time—keeps primary hepatocytes viable and metabolically active for four weeks in culture as measured by CYP activities, albumin secretion, and CYP induction capabilities
• Long-term compound incubation—supports incubations of up to 10 days without medium change, enabling intrinsic clearance studies of low clearance compounds
• Flexible format—delivered frozen; may be aliquoted and refrozen
• Convenient workflow—allows a weekend-free feeding schedule [with media changes every other day (Mon, Wed, Fri) instead of daily] and eliminates complicated overlays or sandwich cultures
• Custom formulations—we offer customization of this product to better serve your research needs. If you`d like a custom formulation, please click here to reach out to our support team and discuss your requirements.

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Applications

• Liver disease modeling
• Hepatotoxicity assessment
• Chronic toxicity
• Intrinsic clearance studies

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.