Takara Ribonuclease H (RNase H)

Takara Ribonuclease H (RNase H)

Applications

• Removal of RNA template following second-strand cDNA synthesis
• Removal of mRNA poly(A) sequence
• Reverse transcription

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.

Concentration

20–60 U/µl

Product Citations

• Schultz, S. J. & Champoux, J. J. RNase H activity: structure, specificity, and function in reverse transcription. Virus Res. 134, 86–103 (2008).
• Vournakis, J. N., Efstratiadis, A. & Kafatos, F. C. Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc. Natl. Acad. Sci. U. S. A. 72, 2959–63 (1975).

Additional Product Information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List to determine kit components.
Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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