Thermo Fisher Scientific CD40 Ligand/CD154/TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (CD40LG, 2761)
CD40 Ligand/CD154 단백질을 인식하는 T세포 활성화 마커용 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 인간 반응성 마우스 IgG2b 항체로, 단백질 A/G 정제 및 PBS 버퍼에 보관. 연구용 전용 제품.
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- 959-MSM1-P0
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD40LG, 2761 |
| Immunogen | Recombinant fragment (around aa 108–261) of human CD40LG protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. It stimulates B cell proliferation and secretion of immunoglobulin isotypes in the presence of cytokines. CD40 Ligand induces cytokine production and tumoricidal activity in peripheral blood monocytes and co-stimulates proliferation of activated T cells, accompanied by the production of IFN-γ, TNF-α, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: 959-MSM1-P0_CD154_CD40_Ligand_P29965-1_House_mouse.svg, 959-MSM1-P0_CD154_CD40_Ligand_P29965-1_House_mouse_PDP.jpeg)
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