Thermo Fisher Scientific DFF45 Monoclonal Antibody (OTI3F12), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

ICAD (Inhibitor of Caspase-Activated DNase) regulates nuclear apoptosis by inhibiting caspase-3-activated DNase (CAD).
It exists in two isoforms:

• ICAD-L/DFF45 (45 kDa): functional isoform
• ICAD-S/DFF35 (35 kDa): non-functional isoform

ICAD is cleaved by caspase-3 at residues 117 and 224, releasing CAD to execute DNA fragmentation during apoptosis.
In non-apoptotic cells, CAD is complexed with ICAD, which also functions as a folding chaperone.
ICAD has been reported as a potential target for restoring normal apoptotic signaling in colon and brain cancer cells.
Two alternatively spliced transcript variants encoding distinct isoforms have been identified for the ICAD gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.