Thermo Fisher Scientific TP53RK Monoclonal Antibody (OTI5A5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Component of the EKC/KEOPS complex required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914).
The complex is involved in transferring the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.
TP53RK exhibits ATPase activity within the EKC/KEOPS complex and supports the catalytic subunit OSGEP.
It is an atypical protein kinase that phosphorylates Ser-15 of p53/TP53, potentially participating in its activation (PubMed:11546806).
[UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.