Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor 570, eBioscience
인간, 마우스, 랫트의 α-평활근 액틴을 인식하는 1A4 단클론 항체로 eFluor 570 형광 표지. 면역조직화학, 세포면역염색, 유세포분석 등에 적합. 556/569 nm 형광 특성, 혈관 및 근육 조직 표지에 유용.
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Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor 570, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | 1:100 | 15 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 9 publications |
| Flow Cytometry (Flow) | - | 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A4 |
| Conjugate | eFluor™ 570 |
| Excitation/Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573630 |
Additional Formats Available:
- Unconjugated
- Alexa Fluor 488
- eFluor 660
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin (α-SMA), a 42 kDa protein that forms part of the cytoskeletal network. This antibody is specific for the alpha isoform expressed by smooth muscle cells, myofibroblasts, and myoepithelial cells. It is useful for identifying leiomyomas, leiomyosarcomas, pleomorphic adenomas, and serves as a prognostic marker for basal cell carcinoma.
Applications Reported:
Used in immunohistochemistry (frozen and paraffin-embedded tissue), microscopy, and immunocytochemistry.
Applications Tested:
Validated for IHC of formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval, effective at ≤1 µg/mL. Optimal concentration should be determined per assay.
Filter Recommendation:
For eFluor™ 570 conjugate, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70). Compatible with Alexa Fluor™ 555 or TRITC filters.
Excitation / Emission:
555 nm / 570 nm
Target Information
Smooth Muscle Actin (α-SMA) is part of the actin protein family involved in cell motility, structure, and integrity.
- Alpha actin: major component of contractile apparatus
- Beta and gamma actins: regulate cell motility
Smooth muscle actin is found in skeletal muscle, vessel walls, gut wall, myometrium, and myoepithelial cells. It interacts with myosin for muscle contraction and cell movement.
Defects in the gene can cause familial thoracic aortic aneurysm type 6.
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle, and also appears in fibroblastic stress fibers during wound healing and fibrocontractive diseases.
For Research Use Only.
Not for diagnostic procedures or resale without authorization.
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