Thermo Fisher Scientific Phospho-c-Jun (Ser63) Monoclonal Antibody (GT653)
Phospho-c-Jun (Ser63)을 인식하는 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합. Human, Rat 시료에 반응하며 단백질 A로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT653 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of c-Jun (phospho Ser63). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735312 |
Product Specific Information
- Positive Control: 293T (25 µg/mL Anisomycin treatment for 30 min)
- Predicted Reactivity: Mouse (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5′-TGA[CG]TCA-3′.
It promotes activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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