Thermo Fisher Scientific Influenza A NP Monoclonal Antibody (D67J), FITC

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:5-1:20

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 8 publications 8 publications

ELISA (ELISA)

1:5-1:20

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

D67J

Immunogen

Influenza A Nucleoprotein.

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 10mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1017747

Product Specific Information

The MA1-7322 antibody reacts with Influenza A virus NP in viral and infected tissue samples. Reactive with all A strains.

MA1-7322 has been successfully used in immunofluorescence, FACS, and ELISA applications. The recommended starting dilution is 1:5 - 1:20 but optimal dilution should be determined by the end user. Acetone fixation of the antigen source is recommended prior to staining.

The MA1-7322 antibody was raised against Influenza A Nucleoprotein.

Target Information

The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56 (Portela et al 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.