Thermo Fisher Scientific H3K18me1 Recombinant Rabbit Monoclonal Antibody (5Q8V4)
H3K18me1에 특이적인 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA, IP, ChIP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 Affinity 정제, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
| Dot blot (DB) | 1:500–1:1,000 |
| CUT&RUN (C&R) | 10⁵ cells / 1 µg |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5Q8V4 |
| Immunogen | Synthetic monomethylated peptide around K18 of human Histone H3 (P68431) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911902 |
Product Specific Information
- Positive test controls: HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein
- Target localization: Chromosome, Nucleus
- Immunogen sequence: APRKQ
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms nucleosomes that package DNA.
Histone H3 is involved in transcription regulation, DNA repair, replication, and chromosomal stability.
Its N-terminal tail undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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