Thermo Fisher Scientific ZSCAN4 Monoclonal Antibody (OTI5A5), TrueMAB
ZSCAN4 단백질을 인식하는 Mouse IgG1 단클론 항체로, 인간 시료에 반응하며 Western blot에 최적화됨. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 배아 발달 및 줄기세포 연구용으로 적합.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A5 |
| Immunogen | Human recombinant protein fragment (aa 164–433 of human ZSCAN4, produced in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Recommended reconstitution: Add 100 µL distilled water to achieve ~1 mg/mL antibody concentration.
- For conjugation experiments, perform additional desalting (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ZSCAN4 is involved in zygotic genome activation (ZGA), the critical transition from maternal to embryonic genetic control. It encodes a SCAN domain and four zinc finger domains, expressed in late 2-cell embryos and embryonic stem cells.
Loss-of-function studies (siRNA) show delayed embryo development from 2-cell to 4-cell stages. ZSCAN4 also contributes to telomere maintenance and genomic stability in ES cells, playing a key role in maintaining stem cell pluripotency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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