Thermo Fisher Scientific ACBD3 Monoclonal Antibody (OTI1G2), TrueMAB
ACBD3 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC(P), Flow Cytometry에 사용 가능. HEK293T 세포에서 생산된 full-length human recombinant immunogen 기반. Lyophilized 형태, -20°C 보관, 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G2 |
| Immunogen | Full length human recombinant protein of human ACBD3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GOLPH1 (also known as GCP60) is a Golgi protein interacting with the integral membrane protein giantin, involved in Golgi structure maintenance. It also interacts with Golgin-160, which can be cleaved by caspases-2, -3, and -7, leading to nuclear localization of Golgin-160 fragments. The interaction strength is regulated by the oxidation state of Cys-463 within GOLPH1.
Additionally, GOLPH1 interacts with Numb, a signaling protein mediating asymmetric cell division of neural progenitor cells, suggesting that Golgi fragmentation and reconstitution during the cell cycle may regulate Numb signaling and couple cell fate with cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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