Thermo Fisher Scientific DOK7 Monoclonal Antibody (OTI7E2), TrueMAB
DOK7 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot, IHC, Flow cytometry에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 시료 반응성이 있으며, 신경근 시냅스 형성과 관련된 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E2 |
| Immunogen | Full length human recombinant protein of human DOK7 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The DOK7 protein is essential for neuromuscular synaptogenesis. It mediates aneural activation of muscle-specific receptor kinase (MuSK), which is required for postsynaptic differentiation and clustering of acetylcholine receptors in myotubes. It can also induce autophosphorylation of MuSK.
Mutations in DOK7 are associated with familial limb-girdle myasthenia (congenital myasthenic syndrome type 1B).
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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