Thermo Fisher Scientific Phospho-c-Cbl (Tyr700) Monoclonal Antibody (M161)
Phospho-c-Cbl (Tyr700) 단백질을 검출하는 Mouse IgG1 단일클론 항체로, Western blot에 최적화되어 있습니다. 인간, 마우스, 랫트 시료에 반응하며, 고순도 Protein A 정제 및 안정적 PBS/BSA/glycerol 저장 buffer 제공. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Phospho-c-Cbl (Tyr700) Monoclonal Antibody (M161)
Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M161 |
| Immunogen | Synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. Highly conserved in rat and mouse c-Cbl. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942601 |
Product Specific Information
The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells.
Target Information
Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation.
Cbl-c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells, whereas other Cbl proteins are ubiquitously expressed.
Cbl-c, like other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation.
Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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