Thermo Fisher Scientific Complement C3 Polyclonal Antibody
Human 및 Mouse 시료에서 Complement C3 단백질 검출용 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 6 publications |
| Immunohistochemistry (IHC) | – | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 4 publications |
| Flow Cytometry (Flow) | – | View 2 publications |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminal region of human C3 (aa 1448–1655) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.07 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153785 |
Product Specific Information
- Recommended positive controls: Huh7, HepG2
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is approximately 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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