Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 인간 및 영장류 시료에 반응하며, Affinity chromatography로 정제됨. PBS/glycerol buffer에 보관하며 -20°C에서 저장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Non-human primate, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human TRIM25 (Accession Q14258), corresponding to amino acid residues A115–E165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850607 |
Product Specific Information
Antibody detects endogenous levels of total ZNF147.
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α. Mice lacking this gene exhibit an underdeveloped uterus and reduced estrogen responsiveness, indicating that this protein is essential for estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, where it promotes tumor growth by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3σ.
Recent studies indicate that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway, which mediates host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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