Norgen Saliva/Swab RNA Purification 96-Well Kit Dx

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비보존 타액 및 비강/인두 면봉에서 고품질 총 RNA를 신속히 정제하는 96웰 형 진단용 키트. CE-IVDR 인증, 고감도·선형성 확보, 바이러스 불활성화 화학 조성 포함. 고처리량 자동화 및 다양한 분자진단 응용에 적합.

카탈로그번호: Dx69300
브랜드: Norgen
카테고리: RNA Kits
판매단위: pk

#Norgen #RNA Reagents #R #Reagents

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Norgen · Norgen Saliva/Swab RNA Purification 96-Well Kit Dx

Norgen Saliva/Swab RNA Purification 96-Well Kit Dx

SKU: Dx69300
For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva.

Features and Benefits

Compatible with preserved saliva collected using Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200)
CE-IVDR marked per European Commission Regulation (EU) No. 2017/746
Integrates seamlessly into in vitro diagnostic workflows
Isolates high-quality total RNA, including viral RNA, from fresh and preserved samples
Rapid and simple spin column processing
Purifies total RNA from large rRNA down to microRNA (miRNA)
No phenol or chloroform extraction required
High sensitivity and linearity down to few cells/viral copies without carrier RNA
Buffer chemistry inactivates viruses including SARS-CoV-2 (Application Note)
Purified RNA suitable for downstream applications such as Small RNA Sequencing (Norgen’s NGS Services)
Utilizes Norgen’s proprietary resin-based spin column chromatography

Product Overview

This kit provides a rapid method for purification of total RNA from non-preserved saliva and nasal/throat swabs, and preserved saliva collected in Norgen’s devices. The proprietary resin selectively purifies RNA from other cellular components without phenol or chloroform. Suitable for medical in vitro diagnostic use.

Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)

Optimized for enzymatic amplification or modification-based downstream assays
High throughput 96-well plate format enables rapid purification
Consistent well-to-well RNA isolation
Binds and elutes all RNA sizes without GC bias
For professional use by trained technicians, physicians, and biologists
Designed for diagnostic workflows; validation required by user

Supporting Data

Figure 1. SARS-CoV-2 E-gene and RdRP Amplification
Linear recovery of spiked SARS-CoV-2 targets demonstrates high sensitivity and RNA quality using Norgen’s 96-Well Kit (Cat. Dx69300).

Figure 2. RNase P Gene Amplification
Fresh and preserved swab/saliva RNA show consistent amplification, confirming RNA integrity.

Figure 3. Human miR-21 Amplification
Detection of miR-21 in both preserved and non-preserved samples validates small RNA recovery performance.

Figure 4. Average RNA Yield
Average yield ≥ 1 µg from preserved and non-preserved samples, indicating robust extraction efficiency.

Kit Specifications

Specification	Description
Sample Volume Range	250 µL
RNA Size Range	All sizes, including small RNA (<200 nt)
Minimum Elution Volume	75 µL
Maximum Elution Volume	100 µL
Time for 96 Purifications	30 minutes
Average Yield	≥ 1 µg (varies by sample)

Storage Conditions and Product Stability

All solutions should be tightly sealed and stored at room temperature.
All components are stable until the expiration date indicated on their labels.

Kit Components

Component	Dx69100 (50 preps)	Dx69300 (192 preps)
Lysis Buffer A	30 mL	100 mL
Solution WN	18 mL	55 mL
Wash Solution A	18 mL	2 × 38 mL
Elution Solution A	6 mL	20 mL
Mini Spin Columns	50	-
96-Well Isolation Plate (Deep Well)	-	2
Collection Tubes	50	-
96-Well Collection Plate (Deep Well)	-	2
Elution Tubes (1.7 mL)	50	-
96-Well Elution Plate (Deep Well)	-	2
Adhesive Tape	-	4
Product Insert	1	1

Automation

Supplementary Protocol:
Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Note:
[Comparing Isolation Efficiency on Hamilton Vantage and QIAsymphony DSP Virus/Pathogen Kit](https://norgenbiotek.com/sites/default/files/resources/App-Note-97-Comparing Isolation Efficiency-0.2.pdf)

Documentation

Protocols

Supplementary Protocols

Automated Procedure Protocol

Application Notes

Safety Data Sheets

Flyers

제품 이미지

Figure 1. SARS-CoV-2 E-gene and RdRP Amplification
Figure 2. RNase P Gene Amplification
Figure 3. Human miR-21 Amplification
Figure 4. Average RNA Yield
Figure 5. SARS-CoV-2 E-gene and RdRP Amplification (96-Well)
Figure 6. RNase P Gene Amplification (96-Well)
Figure 7. Human miR-21 Amplification (96-Well)
Figure 8. Average RNA Yield (96-Well)