Norgen Saliva/Swab RNA Purification 96-Well Kit Dx

Norgen Saliva/Swab RNA Purification 96-Well Kit Dx

SKU: Dx69300
For the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva.

Features and Benefits

• Compatible with preserved saliva collected using Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200)
• CE-IVDR marked per European Commission Regulation (EU) No. 2017/746
• Integrates seamlessly into in vitro diagnostic workflows
• Isolates high-quality total RNA, including viral RNA, from fresh and preserved samples
• Rapid and simple spin column processing
• Purifies total RNA from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extraction required
• High sensitivity and linearity down to few cells/viral copies without carrier RNA
• Buffer chemistry inactivates viruses including SARS-CoV-2 (Application Note)
• Purified RNA suitable for downstream applications such as Small RNA Sequencing (Norgen’s NGS Services)
• Utilizes Norgen’s proprietary resin-based spin column chromatography

Product Overview

This kit provides a rapid method for purification of total RNA from non-preserved saliva and nasal/throat swabs, and preserved saliva collected in Norgen’s devices. The proprietary resin selectively purifies RNA from other cellular components without phenol or chloroform. Suitable for medical in vitro diagnostic use.

Saliva/Swab RNA Purification 96-Well Kit Dx (High Throughput)

• Optimized for enzymatic amplification or modification-based downstream assays
• High throughput 96-well plate format enables rapid purification
• Consistent well-to-well RNA isolation
• Binds and elutes all RNA sizes without GC bias
• For professional use by trained technicians, physicians, and biologists
• Designed for diagnostic workflows; validation required by user

Supporting Data

Figure 1. SARS-CoV-2 E-gene and RdRP Amplification
Linear recovery of spiked SARS-CoV-2 targets demonstrates high sensitivity and RNA quality using Norgen’s 96-Well Kit (Cat. Dx69300).

Figure 2. RNase P Gene Amplification
Fresh and preserved swab/saliva RNA show consistent amplification, confirming RNA integrity.

Figure 3. Human miR-21 Amplification
Detection of miR-21 in both preserved and non-preserved samples validates small RNA recovery performance.

Figure 4. Average RNA Yield
Average yield ≥ 1 µg from preserved and non-preserved samples, indicating robust extraction efficiency.

Kit Specifications

Storage Conditions and Product Stability

All solutions should be tightly sealed and stored at room temperature.
All components are stable until the expiration date indicated on their labels.

Kit Components

Automation

Supplementary Protocol:
Automated Procedure for Saliva/Swab RNA Purification and Concentration

Application Note:
[Comparing Isolation Efficiency on Hamilton Vantage and QIAsymphony DSP Virus/Pathogen Kit](https://norgenbiotek.com/sites/default/files/resources/App-Note-97-Comparing Isolation Efficiency-0.2.pdf)

Documentation

Protocols

• Dx69100 - Saliva/Swab RNA Purification Kit Dx (50 prep)
• Dx69300 - 96-Well Kit Dx (2 × 96-well plates)

Supplementary Protocols

• Automated Procedure Protocol

Application Notes

• Comparison with Magnetic Bead-Based Competitor’s Kit
• Comparison with Zymo’s MagBinding Kit

Safety Data Sheets

• Dx69100 - Spin Column SDS
• Dx69300 - 96-Well Plate SDS

Flyers

• Streamline Your Saliva Workflow
• Saliva/Swab RNA Purification Kits Flyer
• Unlock the Power of Saliva Biomarkers Brochure

Related Products

• Total Nucleic Acid Preservation Tubes Dx
• Saliva RNA Collection and Preservation Devices Dx
• Total RNA Purification Kit Dx
• COVID-19 TaqMan RT-PCR Kit (E/RdRP genes)
• Saliva/Swab RNA Purification Kit Dx (Spin Column)
• 2019-nCoV TaqMan RT-PCR Kit

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