Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 660
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 800
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 690
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation available

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin of the Ig superfamily that binds sialic acid-containing glycoconjugates. Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower expression on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8 but is not expressed on mast cells.

• Recognizes formaldehyde-fixed epitope
• Reported application: Flow cytometric analysis
• Tested on mouse thioglycolate-elicited peritoneal exudate cells (≤0.5 µg/test)
• Recommended titration for optimal performance

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is suitable for UV laser (355 nm) equipped cytometers. Protect samples and reagent from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
For panels using multiple polymer dyes, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic acid-dependent binding. It binds both α2,3- and α2,6-linked sialic acids, with expression in monocytic/myeloid lineage cells. High expression is found in leukocytes, spleen, and bone marrow, lower in lymph node, lung, and other tissues. Expressed by monocytes and neutrophils, absent in early leukemic myelomonocytic cell lines.

For Research Use Only. Not for diagnostic use or resale without authorization.

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