
Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 570, eBioscience
eFluor 570 형광으로 표지된 Mouse IgG1 kappa 동종 대조 항체로, 면역조직화학(IHC) 및 면역세포화학(ICC/IF) 실험에 사용됩니다. 실험 항체의 비특이적 결합을 평가하기 위한 음성 대조용으로 적합하며, 556/569 nm의 여기/방출 특성을 가집니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637442 |
Product Specific Information
Description:
This monoclonal mouse IgG1 κ immunoglobulin serves as an isotype control antibody.
Applications Reported:
Reported for use in immunohistochemical (IHC-P) and immunocytochemical (ICC/IF) staining.
Applications Tested:
Tested by immunocytochemistry on fixed and permeabilized MDCK cells and by immunohistochemistry on formalin-fixed paraffin-embedded human brain tissue.
Use at the same concentration as the experimental antibody.
Filter Recommendation:
For eFluor™ 570 conjugate, use a filter capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Spectral Properties:
Excitation: 555 nm
Emission: 570 nm
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody produced by B cells in response to antigens.
It is the most abundant IgG subtype in serum and plays a key role in the adaptive immune response, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Mouse IgG1 is widely used in research and diagnostics such as ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic studies like immunotherapy and vaccine development.
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Pacific Orange
- Functional Grade
- Custom conjugation available upon request
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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