Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (intracellular staining)
• Tested Dilution: 0.125 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism.
Three isoforms of NOS are encoded by separate genes:

• NOS1 (neuronal NOS, nNOS)
• NOS3 (endothelial NOS, eNOS)
• NOS2 (inducible NOS, iNOS)

NOS2 is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α. It is expressed by myeloid-derived suppressor cells and M1 macrophages, but not M2 macrophages. NOS enzymes are active when forming homodimers, and NOS2 dimerizes at steady-state Ca²⁺ levels.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse thioglycolate-elicited peritoneal exudate cells using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended ≤0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

iNOS (Inducible Nitric Oxide Synthase) mediates NO production from L-arginine to L-citrulline.

• Function: Regulates vasorelaxation, neurotransmission, and cytotoxicity.
• Expression: Inducible in liver and various cells (macrophages, hepatocytes, smooth muscle cells).
• Induction: Stimulated by cytokines (IFN-γ, TNF, IL-1, IL-2) and LPS.
• Isoforms: NOS1 (neuronal), NOS3 (endothelial), NOS2 (inducible).
• Clinical relevance: iNOS dysfunction is associated with achalasia and impotence.

Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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