Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI6H7), TrueMAB
CD80 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, 유세포분석 등에 사용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로 T세포 활성화 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6H7 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain an antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152).
Both are expressed on professional antigen-presenting cells and play essential roles in T cell activation.
CD80 expression is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes.
B7-1 provides regulatory signals for T lymphocytes through binding to CD28 and CTLA4.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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