Thermo Fisher Scientific Phospho-RPS6 (Ser235, Ser236) Polyclonal Antibody
Phospho-RPS6 (Ser235, Ser236) 항체로 인간, 생쥐, 랫트 시료에 반응합니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. KLH 접합 인산화 펩타이드로 면역생성되었으며, Protein A로 정제된 고품질 rabbit polyclonal antibody입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human S6 Ribosomal Protein around phosphorylation site Ser235/236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ribosomes are organelles that catalyze protein synthesis, consisting of a small 40S subunit and a large 60S subunit. These subunits contain 4 RNA species and about 80 distinct proteins. The encoded protein is a cytoplasmic ribosomal protein component of the 40S subunit and belongs to the S6E family. It serves as a major substrate for ribosomal protein kinases, with phosphorylation occurring at several C-terminal serine residues. Phosphorylation is stimulated by growth factors, tumor-promoting agents, and mitogens, while dephosphorylation occurs during growth arrest. This protein may play a role in regulating cell growth and proliferation by selectively translating specific mRNA classes. Multiple processed pseudogenes of this gene exist throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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