Thermo Fisher Scientific NCK1 Recombinant Rabbit Monoclonal Antibody (JU41-80)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Immunoprecipitation (IP)

1:10-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU41-80

Immunogen

Recombinant protein within Human NCK1 aa 204-296 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCK1,uniProtId:P16333-1,ncbiNodeId:9606,antigenRange:204-296,antigenLength:377,antigenImageFileName:MA5-34616_NCK1_P16333-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34616_NCK1_P16333-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848524

Product Specific Information

Positive Control: NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.