Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (002)
Mouse EpCAM/TROP-1/TACSTD1 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. 보존제가 없으며 고순도 Protein A 정제. 높은 특이성과 로트 간 일관성을 제공.
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Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (Clone 002)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (IHC) | - | 2 Publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 | - |
| ELISA | 1:5,000–1:50,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 002 |
| Immunogen | Recombinant Mouse EpCAM/TROP-1/TACSTD1 protein (Met1–Thr266) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785522 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Mouse EpCAM/TROP-1/TACSTD1.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a homotypic, calcium-independent adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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