Thermo Fisher Scientific POLB Polyclonal Antibody
Thermo Fisher Scientific의 POLB 폴리클로날 항체는 인간 POLB 단백질을 인식하는 Rabbit IgG 기반 항체로, Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 연구용 DNA 복구 관련 단백질 분석에 활용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human POLB. Recombinant protein control fragment (Product #RP-103044). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791102 |
Product Specific Information
Immunogen sequence:
YCGVLYFTGS DIFNKNMRAH ALEKGFTINE YTIRPLGVTG VAGEPLPVDS EKDIFDYIQW KYREPKDRSE
Target Information
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain.
The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity, while the C-terminal domain has a nucleotidyltransferase activity.
Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level.
DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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