Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• eFluor 450
• eFluor 615
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure to varying degrees, while lymphocytes are negative.

Applications Reported:
The HI98 antibody is suitable for flow cytometric analysis and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P).

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage ≤ 0.06 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Titrate antibody for optimal performance in specific assays.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression show calcium-dependent self-aggregation, involved in processes like embryo compaction and teratocarcinoma cell aggregation. Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.