Thermo Fisher Scientific Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PerCP-eFluor 710, eBioscience
Phospho-STAT5 (Tyr694) 항체로 인간 및 마우스 세포 내 인산화 STAT5 검출에 적합. PerCP-eFluor 710 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. Blue laser(488 nm) 여기, 710 nm 방출. 고감도, 고특이성 모노클로날 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SRBCZX |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573860 |
Additional Formats
Product Specific Information
Description:
SRBCZX monoclonal antibody recognizes STAT5 phosphorylated on tyrosine 694. STAT proteins are activated by receptor-ligand binding that triggers JAK kinase phosphorylation. Phosphorylated STAT5 translocates to the nucleus to regulate gene transcription in a cytokine-dependent manner.
Cytokines such as IL-2, IL-7, and IL-15 activate STAT5 via JAK1/JAK3, while IL-3, IL-5, and GM-CSF act via JAK2. Phosphorylation at Tyr694 is essential for STAT5 dimerization, nuclear translocation, and DNA binding.
Specificity of SRBCZX clone was validated by ELISA and flow cytometry.
Applications Reported:
Intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for intracellular staining of stimulated human PBMCs. Use 5 µL (0.06 µg) per 100 µL test volume. Recommended cell count: 10⁵–10⁸ cells/test.
Staining Protocol:
Use Protocol C (Two-step: Fixation/Methanol). Protocols A and B are not applicable. Detailed procedures available in BestProtocols® – Staining Intracellular Antigens for Flow Cytometry.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited by the 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable). Protect from light due to photo-oxidation sensitivity.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
STAT5 alpha is a transcription factor activated by cytokines and growth factors. Upon phosphorylation, it forms dimers that translocate to the nucleus to regulate gene expression.
It mediates responses to IL2, IL3, IL7, GM-CSF, erythropoietin, and growth hormones. Constitutive activation (e.g., via TEL/JAK2 fusion) contributes to tumorigenesis.
STAT5 alpha induces BCL2L1/BCL-X(L) expression, conferring anti-apoptotic properties. STAT5 alpha and beta share 96% sequence identity and are co-expressed in many tissues, with differential expression in muscle and mammary glands.
STAT5 alpha is essential in reproduction, lactation, immune function, and growth regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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