Thermo Fisher Scientific CD52 Monoclonal Antibody (CF1D12), Super Bright 600, eBioscience
인간 CD52 단백질을 인식하는 CF1D12 단클론 항체로, Super Bright 600 형광체가 결합된 유세포분석용 항체입니다. 면역세포 분석에 최적화되어 있으며, 405 nm 레이저로 여기되어 600 nm에서 방출합니다. 빛에 민감하며 4°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CF1D12 |
| Immunogen | Human CD52 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762543 |
Additional Formats
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Custom conjugation available
Product Specific Information
This CF1D12 monoclonal antibody reacts with human CD52, a cell surface glycoprotein expressed in immune and reproductive systems. CF1D12 recognizes the carbohydrate region of CD52 rather than the protein core. Tissue-specific glycan modifications may affect epitope recognition. For example, CF1D12 detects human sperm glycoforms of CD52 by western blot but not by flow cytometry.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in 100 µL final volume. Cell count may vary from 10⁵ to 10⁸ cells/test.
Fluorochrome information:
Super Bright 600 is a tandem dye excited with a 405 nm violet laser and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on signal performance.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD52 (also known as CAMPATH-1 antigen) is a glycoprotein expressed on T and B lymphocytes, NK cells, and monocytes. It regulates immune responses and is a target in therapeutic research for multiple sclerosis, chronic lymphocytic leukemia, and autoimmune diseases. CD52 is also the molecular target of alemtuzumab, a monoclonal antibody used for immune cell depletion therapies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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