Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Cyclin B1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고순도(>95%) 보장. 세포주기 G2/M 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 90–140 of Human Cyclin B1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803014 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and one cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded via APC-dependent mechanisms at the end of mitosis. This degradation is necessary for proper cell cycle progression.
Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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