Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• Biotin
• Brilliant UV 615
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 1D11 monoclonal antibody reacts with human NKG2D (CD314), a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, CD8+ T cells, and some CD4+ T cells.
NKG2D associates with the adaptor protein DAP10 and co-stimulates multiple NK activation receptors.
Its counter-receptors MICA/MICB are expressed on various epithelial tumors (lung, breast, kidney, ovary, prostate, colon carcinoma).

Antibodies 5C6 and 1D11 block binding of soluble MICA to γδ TCR T cell clones and inhibit lysis by these cells. They also induce NKG2D-mediated redirected lysis of Fc receptor–bearing P815 cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on normal human peripheral blood cells
  • Use ≤1 µg per test (100 µL final volume)
  • Applicable for 10⁵–10⁸ cells/test (optimize empirically)
  • Excitation: 633–647 nm
  • Emission: 660 nm
  • Laser: Red Laser
  • Filtration: 0.2 µm post-manufacturing filtered

Target Information

Natural killer (NK) cells are lymphocytes capable of lysing tumor and virus-infected cells without prior activation. They regulate both humoral and cell-mediated immunity and express several calcium-dependent (C-type) lectins involved in NK cell function.
The NKG2 gene family, located within the NK complex, encodes type II transmembrane proteins with C-type lectin domains that are preferentially expressed in NK cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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