Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Ultra Violet 661, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: -

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 737
• Brilliant Violet 480, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Red / Violet / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is crucial in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration recommended.

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm; suitable for UV (355 nm) laser-equipped cytometers.

Buffer Recommendation:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Light Sensitivity:
Protect vial and samples from light to prevent photo-induced oxidation.

Excitation / Emission / Laser:
350 nm / 660 nm / Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. Expressed mainly in T helper cells, monocytes, macrophages, and dendritic cells. Detected in thymus, lymph nodes, tonsils, spleen, and select non-lymphoid tissues. CD4 aids early T-cell activation via Lck kinase interaction and may mediate neuronal damage in immune-related CNS diseases. Multiple splice variants identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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