Thermo Fisher Scientific PECAM1 Monoclonal Antibody (OTI2A4), TrueMAB
인간 PECAM1(CD31)을 인식하는 마우스 단클론 항체로, WB, IHC, Flow Cytometry에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 트레할로스 함유 PBS 완충액 기반으로 안정성이 높습니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A4 |
| Immunogen | Full length human recombinant protein of human PECAM1 produced in HEK293T cell (CD31, UniProt ID: P16284-1, range 1–738) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor that regulates T and B cell signaling via immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon phosphorylation, these motifs serve as docking sites for protein-tyrosine phosphatases.
CD31 is widely expressed in the vascular system, primarily at intercellular junctions, and mediates homophilic binding crucial for cell-cell interactions. It plays multiple roles in:
- Integrin-mediated cell adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immune receptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
CD31 is a key regulatory molecule in vascular biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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