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Thermo Fisher Scientific Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin
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Thermo Fisher Scientific Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin

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Rabbit polyclonal secondary antibody recognizing mouse IgG Fc region. Biotin-conjugated for versatile detection in WB, IHC, ICC, ELISA, and Flow Cytometry. High sensitivity and specificity, lyophilized form for stable storage at 4°C. For research use o...

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31813
판매단위
pk
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마지막 업데이트 2025. 08. 05. 오전 07:02
Thermo Fisher Scientific 31813 Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin 1.5 mL pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin

Applications and Tested Dilutions

Application Tested Dilution
Western Blot (WB) 1:20,000–1:400,000
Immunohistochemistry (IHC) 1:500–1:5,000
Immunocytochemistry (ICC/IF) 1:500–1:5,000
Flow Cytometry (Flow) 1:200–1:1,000
ELISA 1:20,000–1:400,000
Immunoprecipitation (IP) 1:500–1:5,000

Product Specifications

Specification Description
Species Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Secondary Antibody
Conjugate Biotin
Form Lyophilized
Concentration 1.6 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.6, with 15 mg/mL BSA
Contains 0.05% sodium azide
Storage Conditions 4°C
Shipping Conditions Ambient
RRID AB_228416
Target IgG Fc
Antibody Form Whole Antibody
Country of Origin USA

Additional Formats

  • [AP conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/AP/host/Rabbit/)
  • [FITC conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/FITC/host/Rabbit/)
  • [HRP conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/HRP/host/Rabbit/)
  • Request custom conjugation

Product Specific Information

  • Concentration may vary slightly between lots; refer to lot-specific datasheet for exact values.
  • Product #31813 has been validated in WB, IF, ICC, IHC, IP, and FACS applications.
  • Reacts with heavy chains of mouse IgG, not with light chains common to most mouse immunoglobulins.
  • Does not react with mouse IgM or non-immunoglobulin serum proteins.
  • May cross-react with immunoglobulins from other species.
  • Store at 4°C. For extended storage, add an equal volume of glycerol (final 50%) and store at -20°C.
  • Reconstitute with 1.5 mL of distilled water before use.

Target Information

Anti-Mouse secondary antibodies are affinity-purified and specifically recognize mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies offer:

  • Increased versatility across detection systems (HRP, AP, fluorescence)
  • Enhanced sensitivity via signal amplification
  • High specificity through affinity purification and conjugation modifications

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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