
Thermo Fisher Scientific Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin
Rabbit polyclonal secondary antibody recognizing mouse IgG Fc region. Biotin-conjugated for versatile detection in WB, IHC, ICC, ELISA, and Flow Cytometry. High sensitivity and specificity, lyophilized form for stable storage at 4°C. For research use o...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:400,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Flow Cytometry (Flow) | 1:200–1:1,000 |
| ELISA | 1:20,000–1:400,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_228416 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
| Country of Origin | USA |
Additional Formats
- [AP conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/AP/host/Rabbit/)
- [FITC conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/FITC/host/Rabbit/)
- [HRP conjugate](/antibody/secondary/product-name/Mouse IgG Fc/conjugates/HRP/host/Rabbit/)
- Request custom conjugation
Product Specific Information
- Concentration may vary slightly between lots; refer to lot-specific datasheet for exact values.
- Product #31813 has been validated in WB, IF, ICC, IHC, IP, and FACS applications.
- Reacts with heavy chains of mouse IgG, not with light chains common to most mouse immunoglobulins.
- Does not react with mouse IgM or non-immunoglobulin serum proteins.
- May cross-react with immunoglobulins from other species.
- Store at 4°C. For extended storage, add an equal volume of glycerol (final 50%) and store at -20°C.
- Reconstitute with 1.5 mL of distilled water before use.
Target Information
Anti-Mouse secondary antibodies are affinity-purified and specifically recognize mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies offer:
- Increased versatility across detection systems (HRP, AP, fluorescence)
- Enhanced sensitivity via signal amplification
- High specificity through affinity purification and conjugation modifications
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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