Thermo Fisher Scientific Horse anti-Mouse IgG (H+L) Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

ELISA (ELISA)

1:200

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Horse / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 3mg/mL BSA

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient

RRID

AB_228365

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 31806 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.

Product # 31806 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. This antibody cross-reacts with mouse IgM (50%) hamster IgG, rat IgG (12%) rat IgM (6%) dog IgG, guinea pig IgG, swine IgG (3%) Rhesus monkey IgG (1%). It has minimal cross-reactivity with immunoglobulins from other species.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.