Thermo Fisher Scientific beta-2 Adaptin Recombinant Rabbit Monoclonal Antibody (9W4U6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9W4U6

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.75 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen Sequence: MEMNFTNKAL QHMTDFAIQF NKNSFGVIPS TPLAIHTPLM PNQSIDVSLP LNTLGPVMKM EPLNNLQVAV KNNIDVFYFS CLIPLNVLFV EDGKMERQVF LATWKDIPNE NELQFQIKEC HLNADTVSSK LQNNNVYTIA KRNVEGQDML YQSLKLTNGI WILAELRIQP GNPNYTLSLK CRAPEVSQYI YQVYDSILKN

Target Information

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate ~100 kDa gene products with similar domain structures (alpha and beta adaptin) and a ~50 and ~17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.