Thermo Fisher Scientific beta-2 Adaptin Recombinant Rabbit Monoclonal Antibody (9W4U6)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

Immunogen Sequence:
MEMNFTNKAL QHMTDFAIQF NKNSFGVIPS TPLAIHTPLM PNQSIDVSLP LNTLGPVMKM EPLNNLQVAV KNNIDVFYFS CLIPLNVLFV EDGKMERQVF LATWKDIPNE NELQFQIKEC HLNADTVSSK LQNNNVYTIA KRNVEGQDML YQSLKLTNGI WILAELRIQP GNPNYTLSLK CRAPEVSQYI YQVYDSILKN

Target Information

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin-coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors, which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors, AP1 and AP2.
AP1 is associated with clathrin-coated vesicles at the trans-Golgi network, while AP2 is associated with endocytic clathrin-coated vesicles at the plasma membrane and interacts with Shc and EGF receptor.
AP2 is composed of four subunits: two separate ~100 kDa gene products (alpha and beta adaptin) with similar domain structures, and ~50 and ~17 kDa subunits. There are two alpha-adaptin genes, alpha A and alpha C, which show tissue-specific expression patterns.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.