Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 760, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 760

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.4 µg)/test

Publications

• [References not provided]

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T-cell development and activation.

OKT8 does not compete with clones RPA-T8 or HIT8a for binding to human peripheral blood leukocytes, indicating distinct epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.4 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor Compatibility:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Blocking Buffer Recommendation:
Use CellBlox Plus Blocking Buffer (Product # C001T06F01) for optimal staining. Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 492 nm
Emission: 766 nm
Laser: 488 nm (Blue)

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family, essential for T-cell signal transduction, differentiation, and activation. CD8 acts as a co-receptor for MHC class I molecules and is expressed on T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide binding, CD8 recruits Lck kinase, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation, crucial for pathogen and tumor clearance.

For Research Use Only. Not for use in diagnostic procedures.

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