Thermo Fisher Scientific Human MAP4K3 (GLK), GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

1-380

Tag

GST-tag

Molecular weight

69.6 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT, 25% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots; aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant human MAP4K3 (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

•Validated in LanthaScreen™ Kinase Binding Assay
•Protocols available for simple use and optimized performance
•Multiple pack sizes available for convenient use

Related Product:
[LanthaScreen™ Eu Kinase Binding Assay](http://www.thermofisher.com/us/en/home/life-science/drug-discovery/target-and-lead-identification-and-validation/kinase biology/kinase-activity-assays/lanthascreentm-eu-kinase-binding-assay.html)

Protein Form: Recombinant, Fragment

The gene accession number for MAP4K3 (GLK) is NP_003609.2.

Target Information

MAP4K3 (also known as GLK) is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. MAP4K3 is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-alpha and can lead to the activation of the JNK signaling pathway. MAP4K3 can directly activate PKC-T, during TCR signaling and people with systemic lupus erythematosus show considerable enhanced MAP4K3 expression and activation of PKC-T, and the kinase IKK in T cells. MAP4K3 can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase 1 (MEKK1). MAP4K3 previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase 3 (MAP4K3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.