Thermo Fisher Scientific Human MAP3K3 (MEKK3), GST Tag Recombinant Protein
인간 MAP3K3(MEKK3) 단백질을 GST 태그로 발현한 재조합 단백질. MAPK 및 ERK 경로 조절 연구에 적합하며, 정제된 액상 형태로 제공. -80°C에서 보관하며 kinase assay 등 다양한 생화학적 분석에 활용 가능.
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Thermo Fisher Scientific Human MAP3K3 (MEKK3), GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | GST-tag |
| Molecular Weight | 99.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris (pH 7.5), 0.5 mM EDTA, 50% glycerol, 0.02% Triton X-100, 150 mM NaCl, 2 mM DTT |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, please spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use material in original packaging until exhausted.
- For larger sizes, aliquoting can reduce freeze/thaw cycles; aliquots below 20 µL are not recommended.
- Do not store kinase diluted.
- Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.
- Protein Form: Full Length
Target Information
MAP3K3 (MEKK3) directly regulates MAPK8 and ERK pathways by phosphorylating multiple MAP2Ks.
Northern blot analysis revealed a 4.6 kb transcript that appears to be ubiquitously expressed.
This protein regulates SAPK and ERK pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway.
In cotransfection assays, it enhances transcription from an NF-κB-dependent reporter gene, indicating a role in the SAPK pathway.
Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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