Thermo Fisher Scientific ATP5B Monoclonal Antibody (OTI6C4), TrueMAB
ATP5B 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot, IHC, ICC, Flow Cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C4 |
| Immunogen | Full length human recombinant protein of human ATP5B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ATP synthase is a highly conserved enzyme found in plants, fungi, bacteria, and animals.
It catalyzes the reversible reaction of ADP and phosphate to form ATP, driven by proton flux across membranes due to electron transfer.
The enzyme consists of two main sections:
- F1 ATP-ase (soluble): alpha, beta, gamma, delta, and epsilon subunits
- F0 ATP-ase (membrane embedded): a, b, and c subunits
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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