Thermo Fisher Scientific ATP5B Monoclonal Antibody (OTI1F7), TrueMAB
ATP5B 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. 고순도 친화 크로마토그래피 정제 및 동결건조 형태. PBS 완충액에 8% 트레할로스 포함, 보존제 무첨가. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F7 |
| Immunogen | Full length human recombinant protein of human ATP5B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ATP synthase is a highly conserved enzyme found in plants, fungi, bacteria, and animals. It catalyzes the reversible conversion of ADP and phosphate to ATP, driven by proton flux across the membrane during electron transfer.
The enzyme consists of two main parts:
- F1 ATP-ase (soluble): composed of alpha, beta, gamma, delta, and epsilon subunits
- F0 ATP-ase (membrane embedded): composed of a, b, and c subunits
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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