Thermo Fisher Scientific Ephrin A5 Polyclonal Antibody
Ephrin A5 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체입니다. Western blot과 IHC(P)에서 사용 가능하며, 인간 시료에 반응합니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 순도를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5 (Gln21–Asn203) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608568 |
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Ephrin-A1, Ephrin-A2, recombinant human Ephrin-A3, Ephrin-A4, Ephrin-B3, and other related proteins is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Ephrin A5 is a cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases crucial for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. It binds promiscuously to Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling. Forward signaling occurs downstream of the receptor, while reverse signaling occurs downstream of the ephrin ligand.
Ephrin A5 activates EPHA3 to regulate cell-cell adhesion and cytoskeletal organization. Interaction with EPHA2 may influence lens fiber cell shape and transparency. It also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion and interacts with EPHA7 to modulate brain development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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