
Thermo Fisher Scientific AK1 Monoclonal Antibody (OTI4A1), TrueMAB
AK1 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 적합. 인간 시료 반응성. 동결건조 형태로 제공되며, PBS 버퍼에 보관. 연구용으로 세포 내 아데닐레이트 키나아제 1 검출에 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A1 |
| Immunogen | Full length human recombinant protein of human AK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Adenylate kinase regulates the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides.
Three isozymes—AK1, AK2, and AK3—exist in vertebrates.
- AK1: Found in cytosol of skeletal muscle, brain, and erythrocytes
- AK2 / AK3: Found in mitochondria of liver and heart tissues
AK1 mutations are associated with nonspherocytic hemolytic anemia due to reduced catalytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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