Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), eFluor 450, eBioscience
FOXP3 단백질을 인식하는 마우스 단클론 항체로, eFluor 450 형광체가 결합되어 있습니다. 인체 및 비인간 영장류 시료에 적합하며, 주로 유세포 분석(Flow Cytometry)에 사용됩니다. Violet 레이저(405 nm)에서 여기되어 445 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 236A/E7 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1548685 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 570
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The 236A/E7 antibody reacts with human FOXP3 protein (also known as FORKHEAD BOX P3, SCURFIN, JM2). Cross-reactivity with other proteins has not been determined. FOXP3 is a 49–55 kDa transcriptional regulator, defective in scurfy mice. It is highly expressed in CD4⁺CD25⁺ regulatory T cells (Treg) and induces Treg phenotype when ectopically expressed in CD4⁺CD25⁻ cells.
Intracellular staining and flow cytometric analysis of freshly isolated human PBMCs using this antibody and the Foxp3 Staining Buffer Set (Product # 00-5523) show staining of the CD4⁺CD25^bright population.
The epitope recognized by 236A/E7 differs from that of PCH101 (Product # 72-5776-40).
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Refer to BestProtocols® for intracellular staining (Protocol B: One-step for nuclear proteins).
Use 5 µL (0.25 µg) per test. A test is defined as antibody amount for a 100 µL sample volume. Cell number may range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited by the 405 nm Violet laser and emitting at 445 nm. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and expressed in CD25⁺CD4⁺ regulatory T cells, low in CD4⁺CD25⁻ cells, and absent in CD4⁻CD8⁺ T cells. FOXP3 acts as a master regulator and specific marker of regulatory T cells.
Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. FOXP3 expression in cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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