Thermo Fisher Scientific CRASP-2 Polyclonal Antibody

Thermo Fisher Scientific CRASP-2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for Borrelia burgdorferi CRASP-2 protein. A BLAST analysis suggests reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. garinii, B. spielmanii, and B. valaisiana sources (91–89% homology). Cross-reactivity with CRASP-2 from other sources has not been determined.

Target Information

CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct manner. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato can evade immune systems to persist in various vertebrate hosts. This activity depends on several factors, including the ability of certain Borrelia species to bind host-derived immune regulators FHL-1 and factor H via CRASPs. Factor H and FHL-1 act as cofactors for factor I, which cleaves complement component 3b (C3b) on the cell surface, conferring resistance to complement-mediated lysis. Discontinuous binding regions may contribute to long-distance interactions with immune regulators, and putative coiled-coil structural elements may play a role in the interaction of B. burgdorferi CRASP-1 with factor H.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.