Thermo Fisher Scientific THEM4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human THEM4. Recombinant protein control fragment (Product #RP-94335). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:THEM4,uniProtId:Q5T1C6-1,ncbiNodeId:9606,antigenRange:147-239,antigenLength:240,antigenImageFileName:PA5-55781_THEM4_Q5T1C6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55781_THEM4_Q5T1C6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2648434

Product Specific Information

Immunogen sequence: PPGFIHGGAI ATMIDATVGM CAMMAGGIVM TANLNINYKR PIPLCSVVMI NSQLDKVEGR KFFVSCNVQS VDEKTLYSEA TSLFIKLNPA KSL

Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 71%.

Target Information

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.