Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (TLD-3A12), PE-Cyanine7, eBioscience
Rat CD31(PECAM-1)을 인식하는 TLD-3A12 단클론 항체로, PE-Cyanine7으로 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 0.25 µg/test 권장, 4°C 암소 보관. 혈관 내피세포 및 백혈구의 CD31 발현 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLD-3A12 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569/780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2573337 |
Available formats:
Product Specific Information
Description:
The TLD-3A12 monoclonal antibody reacts with rat CD31 (PECAM-1, gpIIa), a 130-kDa surface protein highly expressed on endothelial cells and at lower levels on leukocytes and platelets. CD31 mediates homotypic interactions important in adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended ≤0.25 µg per test (defined as staining a 100 µL cell sample). Cell number may range from 10^5 to 10^8 cells/test. Optimal titration is recommended.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Store up to 3 days at 4°C in dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be validated empirically.
Fluorophore Characteristics:
- Excitation: 488–561 nm
- Emission: 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD31 (PECAM-1):
An inhibitory coreceptor regulating T and B cell signaling through ITIM-mediated phosphorylation, providing docking sites for protein-tyrosine phosphatases. Expressed throughout the vascular system, mainly at intercellular junctions. The N-terminal Ig-like domain enables homophilic binding crucial for cell-cell interactions.
CD31 plays roles in:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Immune regulation and autoimmunity
- Macrophage phagocytosis and thrombosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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