Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (tested)
• Recommended dilution: ≤ 0.5 µg/test

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Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 660 / 700
• PE
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available

Product Specific Information

The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with alpha integrins (CD49a–f) to generate VLA-1 through VLA-6 complexes and interacts with CD51.

These integrin heterodimers mediate cellular adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind extracellular matrix proteins (collagen, laminin, fibronectin, vitronectin) and cellular receptors (VCAM-1, MadCAM-1).

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells, ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Light Sensitivity: Protect vial and stained samples from light (photo-induced oxidation).
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29)
A 110 kDa cell surface glycoprotein expressed by various cell types including leukocytes. Forms heterodimers with six alpha chains (α1–α6, CD49a–f) to define VLA integrins. Functions as a cell adhesion molecule and component of complexes binding extracellular matrix proteins.

Decreased ITGB1 expression correlates with multidrug resistance in tumor cells. It plays a critical role in immune cell trafficking, inflammatory response regulation, and tissue remodeling. Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac/skeletal muscle specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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