Thermo Fisher Scientific TRIM25 Recombinant Rabbit Monoclonal Antibody (ARC0971)
TRIM25 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. 인간 시료에 반응하며, HEK293 세포에서 발현되었습니다. 고순도의 친화 크로마토그래피로 정제되어 안정적이며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0971 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 100–200 of human TRIM25 (Q14258) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849389 |
Product Specific Information
Immunogen sequence:
SPNAQVACDH CLKEAAVKTC LVCMASFCQE HLQPHFDSPA FQDHPLQPPV RDLLRRKCSQ HNRLREFFCP EHSECICHIC LVEHKTCSPA SLSQASADLE A
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α. Mice lacking this gene display an underdeveloped uterus and reduced estrogen responsiveness, demonstrating that this protein is essential for estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, promoting tumor growth by functioning as an E3 ubiquitin ligase targeting the negative cell cycle checkpoint 14-3-3σ.
Recent studies indicate that TRIM25 E3 ubiquitin ligase is essential for the RIG-I signaling pathway to elicit host antiviral innate immunity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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