Thermo Fisher Scientific F4/80 Monoclonal Antibody (Cl:A3-1), PE
PE 형광 표지된 Rat monoclonal anti-mouse F4/80 항체로, Flow Cytometry에 적합합니다. 마우스 대식세포 표지에 사용되며, Protein G로 정제된 동결건조 형태입니다. 4°C 암소 보관, 면역원은 C57/BL 마우스 복강 대식세포입니다.
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Thermo Fisher Scientific F4/80 Monoclonal Antibody (Cl:A3-1), PE
Applications
- Flow Cytometry (Flow)
Tested Dilution: Neat
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Cl:A3-1 |
| Immunogen | Thioglycollate-stimulated peritoneal cavity macrophages from C57/BL mice |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA, 5% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538127 |
Additional Formats
- Unconjugated (MA5-16624)
- APC (MA5-16625)
- Biotin (MA5-16626)
- FITC (MA5-16627)
- Custom conjugation available
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Rat anti-mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, an approximately 160 kDa cell surface glycoprotein belonging to the EGF-TM7 family. It shares 68% amino acid identity with human EMR1.
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein, a member of the EGF-TM7 family, sharing 68% identity with human EMR1. Expression varies during macrophage maturation and activation. It is found on mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, peritoneal, lung, thymus, bone marrow, and splenic macrophages. Also expressed on some dendritic cells but absent in T cell zones of spleen and lymph node. Suggested role in generation of efferent CD8+ regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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