Thermo Fisher Scientific NOS3 Monoclonal Antibody (1D12)
인간 eNOS 단백질을 인식하는 마우스 단클론 항체로 Western blot, ELISA, in situ PLA에 사용 가능. 고순도 친화 크로마토그래피 정제 제품으로 PBS 용액 형태. -20°C 보관, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 3 ng/mL |
| in situ PLA | 1:1200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D12 |
| Immunogen | NOS3 (AAH63294.1, 61 a.a. approximately 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
QPPEGPKFPR VKNWEVGSIT YDTLSAQAQQ DGPCTPRRCL GSLVFPRKLQ GRPSPGPPAP EQLLSQARDF INQYYSSIKR SGSQAHEQRL QEVEAEVAAT
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission, and cytotoxicity can all be potentiated through cellular response to NO.
NO production is mediated by members of the nitric oxide synthase (NOS) family, which catalyzes the oxidation of L-arginine to produce L-citrulline and NO.
Three NOS isoforms have been cloned:
- b/nNOS (type I) – neuronal NOS
- eNOS (type III) – endothelial NOS
- iNOS (type II) – inducible NOS
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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