Thermo Fisher Scientific APC1 Polyclonal Antibody
APC1 단백질을 표적으로 하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 적합합니다. 인간 시료 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 주기 관련 단백질 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ANAPC1. Recombinant protein control fragment (Product #RP-102931). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790792 |
Product Specific Information
Immunogen sequence:
DLSKGTQHLK SILSKDGVLY VKLRAGQLSY KEDPMGWQSL LAQTVANRNS EARAFKPETI SAFTSDPALL SFAEYFCKPT VNMGQKQEIL DLFSSV
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis.
APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.
APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence.
The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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